Antigen Analysis: Human 4-1BB ECD Alignment
| ECD Domain | Human | Cyno | Mouse | Rabbit |
|---|---|---|---|---|
| Human Homology | 100% | 95% | 57% | 68% |
High positive hits screening with Beacon
Assay (SA) beads assay
Valid Nano-well: 7560; Positive cell : 932
Positive%: 12.3%
Cell-based assay
Valid Nano-well: 7560; Positive cell : 112
Positive%: 1.48%
NGS sequencing
Validation of recombinant antibodies with ELISA & FACS
31 recombinant
antibodies 
19 clones positive to 4-1BB via ELISA
13 clones positive to human and cyno 4-1BB via FACS
2 clones positive to human, cyno, and mouse 4-1BB via FACS
FACS, Median Fluorescence Intensity (MFI) +/-
Affinity analysis of 4-1BB recombinant antibodies
KD (M) = 9.701E-08
KD (M) = 7.069E-10
