| ￥3500 | |
| Z05648-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 59.64 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized Human NKG2D, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for MICA hFc Chimera, Human, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. »
Immobilized MICA hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-MICA Antibody, hFc Tag with the EC50 of 0.45 μg/ml determined by ELISA. »
The purity of MICA hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
MICA hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
MICA hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 0.36 μM as determined in SPR assay (Biacore T200). »
| Target Background | MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. |
| Synonyms | FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
